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  Indian J Med Microbiol
 

Figure 4: Comparison of indices of oxidative stress injury in sham group, model group, Tt-SOD 100 U g−1 (low-dose) group, Tt-SOD 300 U g−1 (medium-dose) group, and Tt-SOD 500 U g−1 (high-dose) group. (a) Representative images of DCFH-DA fluorescence staining to assess ROS levels in each group; (b) quantitative analysis (green: DCFH-DA, blue: DAPI, 630× magnification). The levels of (c) CAT, (d) SOD, (e) MDA, (f) GSH-PX, and (g) NO in each group were detected using ELISA. Results are expressed as mean ± standard deviation. **P < 0.01, the indicated group versus sham group; #P < 0.05 and ##P < 0.01, the indicated group versus model group. CAT: catalase; DAPI: 4',6-diamidino-2-phenylindole; DCFH-DA: 2',7'-dichlorofluorescein diacetate; ELISA: enzyme-linked immunosorbent assays; GSH-PX: glutathione peroxidase; MDA: malondialdehyde; NO: nitric oxide; SOD: superoxide dismutase; Tt-SOD: Thermus thermophilic-superoxide dismutase.

Figure 4: Comparison of indices of oxidative stress injury in sham group, model group, Tt-SOD 100 U g<sup>−1</sup> (low-dose) group, Tt-SOD 300 U g<sup>−1</sup> (medium-dose) group, and Tt-SOD 500 U g<sup>−1</sup> (high-dose) group. (<b>a</b>) Representative images of DCFH-DA fluorescence staining to assess ROS levels in each group; (<b>b</b>) quantitative analysis (green: DCFH-DA, blue: DAPI, 630× magnification). The levels of (<b>c</b>) CAT, (<b>d</b>) SOD, (<b>e</b>) MDA, (<b>f</b>) GSH-PX, and (<b>g</b>) NO in each group were detected using ELISA. Results are expressed as mean ± standard deviation. <sup>**</sup><i>P</i> < 0.01, the indicated group versus sham group; <sup>#</sup><i>P</i> < 0.05 and <sup>##</sup><i>P</i> < 0.01, the indicated group versus model group. CAT: catalase; DAPI: 4',6-diamidino-2-phenylindole; DCFH-DA: 2',7'-dichlorofluorescein diacetate; ELISA: enzyme-linked immunosorbent assays; GSH-PX: glutathione peroxidase; MDA: malondialdehyde; NO: nitric oxide; SOD: superoxide dismutase; Tt-SOD: Thermus thermophilic-superoxide dismutase.