Figure 2: ACTL7A expression of sperm samples with low and high effective embryo rate. (a) Immunofluorescence with different ACTL7A expression levels in low and high effective embryo rate samples. Green signals represent the signals of ACTL7A and blue signals marked the nucleus by Hoechst33342. Weak signal intensity of ACTL7A were shown in lower samples, while higher samples manifested bright ACTL7A signals in the PT beneath the acrosome. (b) The histogram of gated sperm population of samples with low (n = 5, in blue) and high (n = 5, in red) effective embryo rate. The negative control (in gray) was incubated with preimmune serum. (c) Corresponding western blotting for ACTL7A of samples with low (n = 5) and high (n = 5) effective embryo rate. ACTL7A: actin-like 7A; PT: perinuclear theca.