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  Indian J Med Microbiol
 

Figure 2: Functional analysis ofthe GATA4 p.G64E mutant. (a) Transcriptional activation of claudin-11 promoters activated by GATA4. (b) Transcriptional activation of Star promoters activated by GATA4. HEK293 cells were transiently cotransfected with an empty vector (serving as control) or WT or mutant GATA4 (p.G64E) along with the promoter constructs of (a) claudin-11 and (b) Star. We observed no significant differences in transactivation potential between WT and mutant GATA4 proteins. All promoter activities are reported as fold activation relative to that of the control (vector). (c) HEK293 cells were transfected with WT or p.G64E mutant GATA4. Western blot analysis shows that WT and mutant protein were similarly expressed in total protein extracts. (d) Immunofluorescence staining demonstrated that both WT and mutant GATA4 (green fluorescence) showed strong nuclear localization when expressed in HEK293 cells. The nuclei were stained with DAPI. Scale bar = 25 μm. Results are the mean ± standard deviation of three independent experiments. NS: not significant between two groups. GATA4: GATA-binding protein 4; WT: wild-type; DAPI: 4´,6-diamidino-2-phenylindole.

Figure 2: Functional analysis ofthe <i>GATA4</i> p.G64E mutant. (<b>a</b>) Transcriptional activation of claudin-11 promoters activated by GATA4. (<b>b</b>) Transcriptional activation of <i>Star</i> promoters activated by GATA4. HEK293 cells were transiently cotransfected with an empty vector (serving as control) or WT or mutant GATA4 (p.G64E) along with the promoter constructs of (<b>a</b>) claudin-11 and (<b>b</b>) <i>Star</i>. We observed no significant differences in transactivation potential between WT and mutant GATA4 proteins. All promoter activities are reported as fold activation relative to that of the control (vector). (<b>c</b>) HEK293 cells were transfected with WT or p.G64E mutant GATA4. Western blot analysis shows that WT and mutant protein were similarly expressed in total protein extracts. (<b>d</b>) Immunofluorescence staining demonstrated that both WT and mutant GATA4 (green fluorescence) showed strong nuclear localization when expressed in HEK293 cells. The nuclei were stained with DAPI. Scale bar = 25 μm. Results are the mean ± standard deviation of three independent experiments. NS: not significant between two groups. GATA4: GATA-binding protein 4; WT: wild-type; DAPI: 4´,6-diamidino-2-phenylindole.