Figure 3: Changes in endothelial cells in the corpus cavernosum at 1 week, 3 weeks, and 5 weeks after bilateral cavernous injury. Rat endothelial cell antigen-1 (Reca-1) is a specific marker of endothelial cells. a-SMA is a specific marker of smooth muscle. (a) Representative immunofluorescence images of a-SMA (red) and Reca-1 (green) expression in 5-μm cross sections of cryo-embedded penile midshaft. (b) Comparison of semiquantitative mean densities of Reca- 1 expression between treatment groups at each time point. *P < 0.05 means BCNR and Sham versus the BCNC group; #P < 0.05 means BCNC and Sham versus the BCNR group. (c) Comparison of semi-quantitative mean densities of Reca-1 expression within treatment groups at each time point. *P < 0.05 means week 1 and week 5 versus week 3; #P < 0.05 means week 1 and week 3 versus week 5. BCNR: bilateral cavernous nerve resection; BCNC: bilateral cavernous nerve crushing; α-SMA: alpha-smooth muscle actin; DAPI: 4',6-diamidino-2-phenylindole.