Figure 2: Changes in a-SMA content in the corpus cavernosum at 1 week, 3 weeks, and 5 weeks after bilateral cavernous injury. (a) Representative immunohistochemistry images of α-SMA in 5-μm cross sections of paraffin-embedded penile midshaft. (b) Western blot analysis of a-SMA expression. (c) The ratio of α-SMA to β-actin detection by Western blot. *P < 0.05 means BCNR and Sham versus the BCNC group; #P < 0.05 means BCNC and Sham versus the BCNR group. BCNR: bilateral cavernous nerve resection; BCNC: bilateral cavernous nerve crushing; α-SMA: alpha-smooth muscle actin.