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  Indian J Med Microbiol
 

Figure 2: Alteration in the mRNA expression of piRNA pathway genes in the caput epididymidis of aged Gpx5-/- mice. (a) GSEA analysis result of the piRNA pathway genes. A positive ES with a significant P value and FDR indicates higher mRNA levels of piRNA pathway genes in the WT group and the significant downregulation in the Gpx5-/- mice. (b) RNA-seq data validation by RT-qPCR of six known piRNA pathway genes in the 13-month-old WT and Gpx5-/- groups. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant;*P < 0.05;**P < 0.01; Student's t-test. GSEA: gene set enrichment analysis; ES: enrichment score; FDR: false discovery rate; RT-qPCR: quantitative reverse transcription PCR; Gpx5: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout; NES: normalized enrichment score; piRNA: P-element-induced wimpy testis (PIWI)-interacting RNA.

Figure 2: Alteration in the mRNA expression of piRNA pathway genes in the caput epididymidis of aged <i>Gpx5<sup>-/-</sup></i> mice. (<b>a</b>) GSEA analysis result of the piRNA pathway genes. A positive ES with a significant <i>P</i> value and FDR indicates higher mRNA levels of piRNA pathway genes in the WT group and the significant downregulation in the <i>Gpx5<sup>-/-</sup></i> mice. (<b>b</b>) RNA-seq data validation by RT-qPCR of six known piRNA pathway genes in the 13-month-old WT and <i>Gpx5<sup>-/-</sup></i> groups. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant;<sup>*</sup><i>P</i> < 0.05;<sup>**</sup><i>P</i> < 0.01; Student's <i>t</i>-test. GSEA: gene set enrichment analysis; ES: enrichment score; FDR: false discovery rate; RT-qPCR: quantitative reverse transcription PCR; <i>Gpx5</i>: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout; NES: normalized enrichment score; piRNA: P-element-induced wimpy testis (PIWI)-interacting RNA.