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  Indian J Med Microbiol
 

Figure 1: Analysis of the transcriptomes of the caput epididymidis of aged WT and Gpx5-/- mice. (a) Difference in mRNA expression between the WT and Gpx5-/- groups. Each gene is represented by a dot located according to its expression level between the WT and KO groups. (b) RNA-seq data validation by RT-qPCR of Gpx5 and another three randomly chosen genes in the 13-month-old WT and Gpx5-/- mice. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant;*P < 0.05; Student's t-test. (c) GO enrichment result for the up-regulated genes in the caput epididymis of Gpx5-/- mice. (d) GO enrichment result for the down-regulated genes in the caput epididymis of Gpx5-/- mice. RPKM: reads per kilobase of transcript per million mapped reads; GO: gene ontology; RT-qPCR: quantitative reverse transcription PCR; Gpx5: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout.

Figure 1: Analysis of the transcriptomes of the caput epididymidis of aged WT and Gpx5<sup>-/-</sup> mice. (<b>a</b>) Difference in mRNA expression between the WT and <i>Gpx5<sup>-/-</sup></i> groups. Each gene is represented by a dot located according to its expression level between the WT and KO groups. (<b>b</b>) RNA-seq data validation by RT-qPCR of <i>Gpx5</i> and another three randomly chosen genes in the 13-month-old WT and <i>Gpx5<sup>-/-</sup></i> mice. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant;<sup>*</sup><i>P</i> < 0.05; Student's <i>t</i>-test. (<b>c</b>) GO enrichment result for the up-regulated genes in the caput epididymis of <i>Gpx5<sup>-/-</sup></i> mice. (<b>d</b>) GO enrichment result for the down-regulated genes in the caput epididymis of <i>Gpx5<sup>-/-</sup></i> mice. RPKM: reads per kilobase of transcript per million mapped reads; GO: gene ontology; RT-qPCR: quantitative reverse transcription PCR; <i>Gpx5</i>: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout.