Figure 2: PHB interacts with AKT and exists in a complex with phospho-PHB (pT258) and phospho-AKT (pT308) in mouse mature sperm. The interaction of PHB with AKT in mouse cauda epididymal sperm was determined using coimmunoprecipitation analysis. A total lysate indicated as input was used as a positive control while a lysate with IgG treatment was used as a negative control. After co-IP with a PHB antibody, PHB was found to bind with AKT in a complex, in which phospho-PHB (pT258) and phospho-AKT (pT308) were present as well. PHB: prohibitin; AKT: protein kinase B; IP: immunoprecipitation.