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  Indian J Med Microbiol
 

Figure 1: Bull sperm subpopulations stained with ISAS®3Fun (1), and their mask obtained after processing with an ImageJ plug-in module (2–4). Spermatozoa with (a) intact acrosome and membrane; (b) intact acrosome and damaged membrane; (c) damaged acrosome and intact membrane; and (d) damaged acrosome and membrane. The software automatically analyzes the sperm head (2), nucleus (3), and acrosome (4) and provides morphometric measurements. Scale bar= 5 μm.

Figure 1: Bull sperm subpopulations stained with ISAS<sup>®</sup>3Fun (1), and their mask obtained after processing with an ImageJ plug-in module (2–4). Spermatozoa with (<b>a</b>) intact acrosome and membrane; (<b>b</b>) intact acrosome and damaged membrane; (<b>c</b>) damaged acrosome and intact membrane; and (d) damaged acrosome and membrane. The software automatically analyzes the sperm head (2), nucleus (3), and acrosome (4) and provides morphometric measurements. Scale bar= 5 μm.