Figure 2: Expression of PK2 and PKR1 in varicocele. (a) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. (b) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. (c) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, **P < 0.01 (t-test). PK2: prokineticin 2; PKR1: prokineticin receptor 1; qPCR: quantitative real-time PCR assays; Con: control group; VC: varicocele.