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  Indian J Med Microbiol
 

Figure 4: Assessment of MFI and percentage of labeled cells in the sperm populations labeled with MitoPY1 and DAF-2 DA. (a–c) MFI values were obtained through FC for MitoPY1 and compared to the positive controls: (a) MPY1 versus MPY1 + AA (n = 28), (b) MPY1 versus MPY1 + H2O2 (n = 30) and (c) MPY1 versus MPY1 + H2O2 + EDTA (n = 14); (d–f) PLC values were obtained through FC for MitoPY1 and compared to the positive controls: (d) MPY1 versus MPY1 + AA (n = 28), (e) MPY1 versus MPY1 + H2O2 (n = 30) and (f) MitoPY1 versus MPY1 + H2O2 + EDTA (n = 14); (g) MFI and (h) PLC values were obtained through FC for DAF-2 DA and compared to the positive control: (g) D2D versus D2D + Spermine (n = 28), (h) D2D versus D2D + Spermine (n = 28). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. ***P < 0.001. MPY1: MitoPY1; D2D: DAF-2 DA; AA: antimycin A; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.

Figure 4: Assessment of MFI and percentage of labeled cells in the sperm populations labeled with MitoPY1 and DAF-2 DA. (<b>a–c</b>) MFI values were obtained through FC for MitoPY1 and compared to the positive controls: (<b>a</b>) MPY1 versus MPY1 + AA (<i>n = </i>28), (<b>b</b>) MPY1 versus MPY1 + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>30) and (<b>c</b>) MPY1 versus MPY1 + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>14); (<b>d–f</b>)<b> </b>PLC values were obtained through FC for MitoPY1 and compared to the positive controls: (<b>d</b>) MPY1 versus MPY1 + AA (<i>n = </i>28), (<b>e</b>) MPY1 versus MPY1 + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>30) and (<b>f</b>) MitoPY1 versus MPY1 + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>14); (<b>g</b>) MFI and (<b>h</b>) PLC values were obtained through FC for DAF-2 DA and compared to the positive control: (<b>g</b>) D2D versus D2D + Spermine (<i>n = </i>28), (<b>h</b>) D2D versus D2D + Spermine (<i>n = </i>28). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. <sup>***</sup><i>P </i>< 0.001. MPY1: MitoPY1; D2D: DAF-2 DA; AA: antimycin A; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.