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  Indian J Med Microbiol
 

Figure 3: Assessment of MFI and PLC in the sperm populations labeled with RedoxSensor™ Red CC-1 and CellROX® Orange Reagent. (a–c) MFI values were obtained through FC for RedoxSensor™ Red CC-1 and compared to the positive controls: (a) RSR CC-1 versus RSR + AA (n = 29), (b) RSR versus RSR + H2O2 (n = 31) and (c) RSR versus RSR + H2O2 + EDTA (n = 18); (d–f) PLC values were obtained through FC for RedoxSensor™ Red CC-1 and compared to the positive controls: (d) RSR versus RSR + AA (n = 29), (e) RSR versus RSR + H2O2 (n = 31) and (f) RSR versus RSR + H2O2 + EDTA (n = 18); (g–i) MFI values were obtained through FC for CellROX® Orange Reagent and compared to the positive controls: (g) CRO versus CRO + AA (n = 31), (h) CRO versus CRO + H2O2 (n = 33) and (i) CRO versus CRO + H2O2 + EDTA (n = 13); (j–l) PLC values were obtained through FC for CRO and compared to the positive controls: (j) CRO versus CRO + AA (n = 31), (k) CRO versus CRO + H2O2 (n = 33) and (l) CRO versus CRO + H2O2 + EDTA (n = 13). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. **P < 0.01, ***P < 0.001. RSR: RedoxSensor™ Red CC-1; CRO: CellROX® Orange Reagent; AA: antimycin A; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.

Figure 3: Assessment of MFI and PLC in the sperm populations labeled with RedoxSensor™ Red CC-1 and CellROX<sup>®</sup> Orange Reagent. (<b>a–c</b>)<b> </b>MFI values were obtained through FC for RedoxSensor™ Red CC-1 and compared to the positive controls: (<b>a</b>) RSR CC-1 versus RSR + AA (<i>n = </i>29), (<b>b</b>) RSR versus RSR + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>31) and (<b>c</b>) RSR versus RSR + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>18); (<b>d–f</b>)<b> </b>PLC values were obtained through FC for RedoxSensor™ Red CC-1 and compared to the positive controls: (<b>d</b>) RSR versus RSR + AA (<i>n = </i>29), (<b>e</b>) RSR versus RSR + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>31) and (<b>f</b>) RSR versus RSR + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>18); (<b>g–i</b>)<b> </b>MFI values were obtained through FC for CellROX<sup>®</sup> Orange Reagent and compared to the positive controls: (<b>g</b>) CRO versus CRO + AA (<i>n = </i>31), (<b>h</b>) CRO versus CRO + H<sub>2</sub>O<sub>2</sub><b> </b>(<i>n = </i>33) and (<b>i</b>) CRO versus CRO + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>13); (<b>j–l</b>)<b> </b>PLC values were obtained through FC for CRO and compared to the positive controls: (<b>j</b>) CRO versus CRO + AA (<i>n = </i>31), (<b>k</b>) CRO versus CRO + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>33) and (<b>l</b>) CRO versus CRO + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>13). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. <sup>**</sup><i>P </i>< 0.01, <sup>***</sup><i>P </i>< 0.001. RSR: RedoxSensor™ Red CC-1; CRO: CellROX<sup>®</sup> Orange Reagent; AA: antimycin A; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.