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  Indian J Med Microbiol
 

Figure 2: Assessment of MFI and PLC in the sperm populations labeled with MitoSOX™ Red and DHE. (a–c) MFI values were obtained through FC for MitoSOX™ Red and compared to the positive controls: (a) MSR versus MSR + AA (n = 33), (b) MSR versus MSR + H2O2 (n = 31) and (c) MSR versus MSR + H2O2 + EDTA (n = 15); (d–f) PLC values were obtained through FC for MitoSOX™ Red and compared to the positive controls: (d) MSR versus MSR + AA (n = 33), (e) MSR versus MSR + H2O2 (n = 31) and (f) MSR versus MSR + H2O2 + EDTA (n = 15); (g–i) MFI values were obtained through FC for DHE and compared to the positive controls: (g) DHE versus DHE + AA (n = 37), (h) DHE versus DHE + H2O2 (n = 36) and (i) DHE versus DHE + H2O2 + EDTA (n = 19); (j–l) PLC values were obtained through FC for DHE and compared to the positive controls: (j) DHE versus DHE + AA (n = 37), (k) DHE versus DHE + H2O2 (n = 36) and (l) DHE versus DHE + H2O2 + EDTA (n = 19). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. **P < 0.01, ***P < 0.001. MSR: MitoSOX™ Red; AA: antimycin A; DHE: dihydroethidium; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.

Figure 2: Assessment of MFI and PLC in the sperm populations labeled with MitoSOX™ Red and DHE. (<b>a–c</b>) MFI values were obtained through FC for MitoSOX™ Red and compared to the positive controls: (<b>a</b>) MSR versus<i> </i>MSR + AA (<i>n = </i>33), (<b>b</b>) MSR<i> </i>versus<i> </i>MSR + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>31) and (<b>c</b>) MSR versus MSR + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>15); (<b>d–f</b>)<b> </b>PLC values were obtained through FC for MitoSOX™ Red and compared to the positive controls: (<b>d</b>) MSR versus MSR + AA (<i>n = </i>33), (<b>e</b>) MSR versus MSR + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>31) and (<b>f</b>) MSR versus MSR + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>15); (<b>g–i</b>)<b> </b>MFI values were obtained through FC for DHE and compared to the positive controls: (<b>g</b>) DHE versus DHE + AA (<i>n = </i>37), (<b>h</b>) DHE versus DHE + H<sub>2</sub>O<sub>2</sub><i> </i>(<i>n = </i>36) and (<b>i</b>) DHE versus DHE + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>19); (<b>j–l</b>)<b> </b>PLC values were obtained through FC for DHE and compared to the positive controls: (<b>j</b>) DHE versus DHE + AA (<i>n = </i>37), (<b>k</b>) DHE versus DHE + H<sub>2</sub>O<sub>2</sub> (<i>n = </i>36) and (<b>l</b>) DHE versus DHE + H<sub>2</sub>O<sub>2</sub> + EDTA (<i>n = </i>19). All data present a non-Gaussian distribution; therefore, the Wilcoxon test was performed. Data are presented as quartiles, Q1, Q2 (median) and Q3. <sup>**</sup><i>P </i>< 0.01, <sup>***</sup><i>P </i>< 0.001. MSR: MitoSOX™ Red; AA: antimycin A; DHE: dihydroethidium; FC: flow cytometry; EDTA: ethylenediamine tetra acetic acid; MFI: mean fluorescence intensity; PLC: percentage of labeled cells.