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  Indian J Med Microbiol
 

Figure 5: Co-expression profile of GFRA1/PLZF in whole mount IF. GFRA1 was co-located with strong PLZF expression in As, Apr, and Aal4 undifferentiated spermatogonia throughout the seminiferous cycle. No GFRA1 was detected co-staining with (a) PLZFHIGH A4 in stage I, (b) PLZFNEG In in stage III, (c) PLZFNEG B in stage V, (d) PLZFLOW A1 in stage VII, (e) PLZFLOW A2 in stage X and (f) PLZFLOW A3 in stage XII in cross section and whole mount IHC. Indicators of multiple germ cell types were the same as in Co-expression profile of GFRA1/PLZF in whole mount IF. GFRA1 was co-located with strong PLZF expression in As, Apr, and Aal4 undifferentiated spermatogonia throughout the seminiferous cycle. No GFRA1 was detected co-staining with (a) PLZFHIGH A4 in stage I, (b) PLZFNEG In in stage III, (c) PLZFNEG B in stage V, (d) PLZFLOW A1 in stage VII, (e) PLZFLOW A2 in stage X and (f) PLZFLOW A3 in stage XII in cross section and whole mount IHC. Indicators of multiple germ cell types were the same as in figure 4. Scale bar = 25 um.

Figure 5: Co-expression profile of GFRA1/PLZF in whole mount IF. GFRA1 was co-located with strong PLZF expression in A<sub>s</sub>, A<sub>pr</sub>, and A<sub>al4</sub> undifferentiated spermatogonia throughout the seminiferous cycle. No GFRA1 was detected co-staining with (<b>a</b>) PLZF<sup>HIGH</sup> A<sub>4</sub> in stage I, (<b>b</b>) PLZF<sup>NEG</sup> In in stage III, (<b>c</b>) PLZF<sup>NEG</sup> B in stage V, (<b>d</b>) PLZF<sup>LOW</sup> A<sub>1</sub> in stage VII, (<b>e</b>) PLZF<sup>LOW</sup> A<sub>2</sub> in stage X and (<b>f</b>) PLZF<sup>LOW</sup> A<sub>3</sub> in stage XII in cross section and whole mount IHC. Indicators of multiple germ cell types were the same as in Co-expression profile of GFRA1/PLZF in whole mount IF. GFRA1 was co-located with strong PLZF expression in A<sub>s</sub>, A<sub>pr</sub>, and A<sub>al4</sub> undifferentiated spermatogonia throughout the seminiferous cycle. No GFRA1 was detected co-staining with (a) PLZF<sup>HIGH</sup> A4 in stage I, (b) PLZF<sup>NEG</sup> In in stage III, (c) PLZF<sup>NEG</sup> B in stage V, (d) PLZF<sup>LOW</sup> A<sub>1</sub> in stage VII, (e) PLZF<sup>LOW</sup> A<sub>2</sub> in stage X and (f) PLZF<sup>LOW</sup> A<sub>3</sub> in stage XII in cross section and whole mount IHC. Indicators of multiple germ cell types were the same as in figure 4. Scale bar = 25 um.