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  Indian J Med Microbiol
 

Figure 4: Co-expression profile of c-KIT/PLZF in whole mount IF. (a) A4 was c-KITPOSPLZFLOW in stage I, (b) In was c-KITPOSPLZFNEG in stage III, (c) B was c-KITPOSPLZFNEG in stage V, (d) A1 was c-KITPOSPLZFHIGH in stage VII, (e) A2 was c-KITPOSPLZFLOW in stage X, (f) A3 was c-KITPOSPLZFLOW in stage XII in cross section and whole mount IHC. no c-KIT was detected co-staining with PLZFHIGH undifferentiated A spermatogonia throughout the seminiferous cycle. Aun: white arrows; A1: red arrows; A2: green arrows; A3: yellow arrows; A4: pink arrows; In: grey arrows; B: cyan arrows; preleptotene: white asterisks; leptotene: red asterisks; zygotene: green asterisks; early pachytene: yellow asterisks; mid-pachytene: pink asterisks; late pachytene: grey asterisks; meiotic division: cyan asterisks; Sertoli cells- white arrowheads; round spermatid: white triangles; elongating spermatid: red triangles. Scale bar=25 μm.

Figure 4: Co-expression profile of c-KIT/PLZF in whole mount IF. (<b>a</b>) A<sub>4</sub> was c-KIT<sup>POS</sup>PLZF<sup>LOW</sup> in stage I, (<b>b</b>) In was c-KIT<sup>POS</sup>PLZF<sup>NEG</sup> in stage III, (<b>c</b>) B was c-KIT<sup>POS</sup>PLZF<sup>NEG</sup> in stage V, (<b>d</b>) A<sub>1</sub> was c-KIT<sup>POS</sup>PLZF<sup>HIGH</sup> in stage VII, (<b>e</b>) A<sub>2</sub> was c-KIT<sup>POS</sup>PLZF<sup>LOW</sup> in stage X, (<b>f</b>) A<sub>3</sub> was c-KIT<sup>POS</sup>PLZF<sup>LOW</sup> in stage XII in cross section and whole mount IHC. no c-KIT was detected co-staining with PLZF<sup>HIGH</sup> undifferentiated A spermatogonia throughout the seminiferous cycle. A<sub>un</sub>: white arrows; A<sub>1</sub>: red arrows; A<sub>2</sub>: green arrows; A<sub>3</sub>: yellow arrows; A<sub>4</sub>: pink arrows; In: grey arrows; B: cyan arrows; preleptotene: white asterisks; leptotene: red asterisks; zygotene: green asterisks; early pachytene: yellow asterisks; mid-pachytene: pink asterisks; late pachytene: grey asterisks; meiotic division: cyan asterisks; Sertoli cells- white arrowheads; round spermatid: white triangles; elongating spermatid: red triangles. Scale bar=25 μm.