Figure 2: Photomicrographs showing (a) the seminiferous tubule diameter (×100, scale bars=100 μm), (b) the seminiferous epithelium height (×200, scale bars=100 μm), and (c) the proliferation of a spermatogenic cell line (×400, scale bars=50 μm) in animals at 5 months of age. The seminiferous tubule diameter was similar among the groups. However, CAF diet reduced the height of the seminiferous epithelium and the proliferation of a spermatogenic cell line in relation to C, which may affect sperm production. The treatment of resveratrol attenuated this damage in proliferative cells, as observed in the anti-PCNA antibody-immunolabeled image. PCNA: proliferating cell nuclear antigen. C: control diet; CAF: cafeteria diet; C-R: control diet treated with resveratrol; CAF-R: cafeteria diet treated with resveratrol.