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  Indian J Med Microbiol
 

Figure 1: Comparison of Kova and improved Neubauer for sperm concentration measurement. A total of 60 different sperm concentrations were measured with the two methods. (a) Bland–Altman plot of difference in the concentration (106 ml−1) measured with Kova chamber and improved Neubauer hemocytometer against the mean of the 2 values (circles). The solid line represents the mean of differences. The dotted lines indicate the 95% confidence limits (mean ± 1.96 × s.d.). (b) Linear regression of the log-transformed concentrations (106 ml−1) estimated with Kova chamber 10 (y-axis) and improved Neubauer hemocytometer (x-axis). The solid line represents the Tessier's linear regression (XK = 0.95XN + 0.94).

Figure 1: Comparison of Kova and improved Neubauer for sperm concentration measurement. A total of 60 different sperm concentrations were measured with the two methods. (<b>a</b>) Bland–Altman plot of difference in the concentration (10<sup>6</sup> ml<sup>−1</sup>) measured with Kova chamber and improved Neubauer hemocytometer against the mean of the 2 values (circles). The solid line represents the mean of differences. The dotted lines indicate the 95% confidence limits (mean ± 1.96 × s.d.). (<b>b</b>) Linear regression of the log-transformed concentrations (10<sup>6</sup> ml<sup>−1</sup>) estimated with Kova chamber 10 (y-axis) and improved Neubauer hemocytometer (x-axis). The solid line represents the Tessier's linear regression (X<sub>K</sub> = 0.95X<sub>N</sub> + 0.94).