Close
  Indian J Med Microbiol
 

Figure 6: Combination therapy with SVF and Ad-COMP-Ang1 gene increases penile nNOS and neurofilament content in STZ-induced diabetic mice and enhances neurite sprouting in an ex vivo MPG tissue culture. (a) Anti-nNOS (red) and (b) anti-neurofilament (NF; red) staining in cavernous tissue from age-matched control mice (C) group and STZ-induced diabetic mice stimulated at 4 weeks after intracavernous injection of PBS (P, 20 μl), ad-LacZ (L, 2 × 108 particles per 20 μl), SVF (S, 1 × 105 cells per 20 μl), Ad-COMP-Ang1 (CA, 2 × 108 particles per 20 μl), or combination (S + CA). Nuclei were labeled with DAPI (blue). Scale bars = 50 μm (cavernosum) or 25 μm (dorsal nerve). (c) Neurofilament (red) staining in MPG-cultured tissue. Scale bars = 200 μm. Quantification of the (d) nNOS and (e) NF immunopositive areas in cavernous tissue by ImageJ. Each bar depicts the mean value ± standard deviation from n = 5 animals per group. *P < 0.001, P or L versus C group. #P < 0.001, S or CA versus P or L groups. P < 0.05, S + CA versus S or CA groups. (f) Quantification of neurite length by ImageJ. Each bar depicts the mean values ±standard deviations from n = 4 animals per group. *P < 0.001, P or L versus C group. #P < 0.001, S or CA versus P or L groups. P < 0.001, S + CA versus S or CA groups. The P values were determined by one-way ANOVA. Ad-COMP-Ang1: adenovirus-mediated cartilage oligomeric matrix angiopoietin-1; DAPI: 4',6-diamidino-2-phenylindole; PBS: phosphate-buffered saline; nNOS: neuronal nitric oxide synthase; STZ: streptozotocin; SVF: stromal vascular fraction; MPG: major pelvic ganglion.

Figure 6: Combination therapy with SVF and <i>Ad-COMP-Ang1</i> gene increases penile nNOS and neurofilament content in STZ-induced diabetic mice and enhances neurite sprouting in an <i>ex vivo</i> MPG tissue culture. (<b>a</b>) Anti-nNOS (red) and (<b>b</b>) anti-neurofilament (NF; red) staining in cavernous tissue from age-matched control mice (C) group and STZ-induced diabetic mice stimulated at 4 weeks after intracavernous injection of PBS (P, 20 μl), ad-LacZ (L, 2 × 10<sup>8</sup> particles per 20 μl), SVF (S, 1 × 10<sup>5</sup> cells per 20 μl), <i>Ad-COMP-Ang1</i> (CA, 2 × 10<sup>8</sup> particles per 20 μl), or combination (S + CA). Nuclei were labeled with DAPI (blue). Scale bars = 50 μm (cavernosum) or 25 μm (dorsal nerve). (<b>c</b>) Neurofilament (red) staining in MPG-cultured tissue. Scale bars = 200 μm. Quantification of the (<b>d</b>) nNOS and (<b>e</b>) NF immunopositive areas in cavernous tissue by ImageJ. Each bar depicts the mean value ± standard deviation from <i>n</i> = 5 animals per group. <sup>*</sup><i>P</i> < 0.001, <i>P</i> or L versus C group. <sup>#</sup><i>P</i> < 0.001, S or CA versus <i>P</i> or L groups. <sup>†</sup><i>P</i> < 0.05, S + CA versus S or CA groups. (<b>f</b>) Quantification of neurite length by ImageJ. Each bar depicts the mean values ±standard deviations from <i>n</i> = 4 animals per group. <sup>*</sup><i>P</i> < 0.001, <i>P</i> or L versus C group. <sup>#</sup><i>P</i> < 0.001, S or CA versus <i>P</i> or L groups. <sup>†</sup><i>P</i> < 0.001, S + CA versus S or CA groups. The <i>P</i> values were determined by one-way ANOVA. <i>Ad-COMP-Ang1</i>: adenovirus-mediated cartilage oligomeric matrix angiopoietin-1; DAPI: 4',6-diamidino-2-phenylindole; PBS: phosphate-buffered saline; nNOS: neuronal nitric oxide synthase; STZ: streptozotocin; SVF: stromal vascular fraction; MPG: major pelvic ganglion.