Figure 5: nNOS expression in dorsal penile nerve and major pelvic ganglia. (a) Representative images of nNOS expression in the dorsal penile nerve (DPN) and nNOS-positive neurons in major pelvic ganglia (MPG), original magnification: ×200. Graph summarizing the quantitative data of nNOS content in DPN (b) and nNOS-positive cells in MPG (c). (d) Western blot analysis of nNOS and NGF contents in penis. Protein levels are quantified by nNOS/GAPDH (e) and NGF/GAPDH (f) in each column and expressed as mean ± standard deviation. *P < 0.05 compared with DM + PBS group. &P < 0.05 compared with DM + ADSCs group.