Figure 2: Venn diagram showing a total of 1469 proteins in the pooled samples. 1856 and 1650 proteins were found in two individual samples respectively (a). Of these proteins, 1207 proteins were found in both pooled and individual samples. Of the 525 proteins found only in individual sample 1 but not in pooled sample, (b) Western blotting showing HSP 70 1A, Clusterin, Tektin 2, and Tektin 3 β-actin in four fractions (n = 4), ( c-f ) trend in protein expression in four fractions. Error bars represent standard deviations values for each parameter.