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  Indian J Med Microbiol
 

Figure 1: Macrosurgical epididymal sperm collection procedure. (a) The epididymal tunic was incised and epididymal tubules in the proximal portion of the obstructed epididymis were exposed. (b) One of the epididymal tubules was cut with a 24-gauge injection needle without isolation. (c) Epididymal fluid (red arrow) emerging from the incised epididymal tubule was harvested simply by (d) touching the base of disposable plastic dishes directly to the incised epididymal tubule.

Figure 1: Macrosurgical epididymal sperm collection procedure. (a) The epididymal tunic was incised and epididymal tubules in the proximal portion of the obstructed epididymis were exposed. (b) One of the epididymal tubules was cut with a 24-gauge injection needle without isolation. (c) Epididymal fluid (red arrow) emerging from the incised epididymal tubule was harvested simply by (d) touching the base of disposable plastic dishes directly to the incised epididymal tubule.