Figure 2: The effect of extracellular polymeric substances (EPS) of Trichomonas vaginalis on the viability of sperm. Viability was analyzed based on sperm morphology and vital staining with trypan blue. Data are presented at the mean ± standard deviation percent of viable sperm (relative to the total sperm numbers set at 100%) in three separate experiments, in which 200 cells were counted per treatment per experiment. EPS-10, -25, -50, -75, -100: sperm cells were incubated for 1, 2, or 3 h in 10%, 25%, 50%, 75%, or 100% of EPS prepared from T. vaginalis were diluted in Hank's balanced salt solution (HBSS). CT: control sperm cells were incubated at 37°C for 1, 2, or 3 h in HBSS that did not contain EPS.