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  Indian J Med Microbiol
 

Figure 4: Examination of androgen-related histological changes in the bladder and blood vessels. ( a ) Smooth muscle/collagen ratio: bladder. ( b ) Smooth muscle/collagen ratio: common iliac artery. The bladders and iliac arteries of rats were histologically examined for differences in smooth muscle and the quantity of collagen fiber to analyze the effect of castration on the smooth muscle content. We used Mallory-stained specimens for the examination of histological changes such as denaturation or fibrosis. Sections of stained tissues were observed at a magnification of ×400 under a light microscope. The components of smooth muscles and connective tissues were calculated from at least 10 fields from each tissue section.

Figure 4: Examination of androgen-related histological changes in the bladder and blood vessels. ( <b>a</b> ) Smooth muscle/collagen ratio: bladder. ( <b>b</b> ) Smooth muscle/collagen ratio: common iliac artery. The bladders and iliac arteries of rats were histologically examined for differences in smooth muscle and the quantity of collagen fiber to analyze the effect of castration on the smooth muscle content. We used Mallory-stained specimens for the examination of histological changes such as denaturation or fibrosis. Sections of stained tissues were observed at a magnification of ×400 under a light microscope. The components of smooth muscles and connective tissues were calculated from at least 10 fields from each tissue section.