Figure 1: Androgen-related changes in BBF (not significant). The left carotid and left femoral arteries of rats were cannulated. A total of 0.2 ml of a yellow-green microsphere was injected through the carotid artery cannula and was flushed with 0.7 ml of saline to equalize the injecting and withdrawing volumes. The microspheres were extracted from the removed bladder and the collected blood according to the manufacturer's instructions. The concentration of fluorescent dye was measured in triplicate using a microplate reader at the following wavelengths: excitation wavelength, 490 nm; emission wavelength, 530 nm; and bandwidth, 5 nm.