Figure 3: DNA methyltransferases inhibitors could recover the expression of SRD5A2 mRNA in BPH-1 cells. (a) SRD5A2 mRNA was detected in BPE tissue but was not expressed in BPH-1 cells or some malignant epithelial cells. (b) SRD5A2 mRNA was re-expressed in BPH-1 cells that were treated with 5-Aza-CdR or RG108. “M” represents the molecular weight standard. Control: negative control without the loading of SRD5A2 mRNA. BPE: benign prostatic enlargement; SRD5A2: 5-α reductase type 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; 5-Aza-CdR: 5-Aza-2'-deoxycytidine; RG108: N-Phthalyl-L-tryptophan; HAC: acetic acid; DMSO: dimethyl sulfoxide.