Figure 1: A total of forty BPE specimens were evaluated for immunoreactivity to SRD5A2 and DNMT1. (a) SRD5A2 was mostly expressed in epithelial cells and showed broad expression in both the cytoplasm and nuclei. DNMT1 was mainly expressed in the nuclei of epithelial cells. Sample 1: strong SRD5A2 expression with corresponding reduced expression of DNMT1. Sample 2: negative SRD5A2 expression with corresponding strong expression of DNMT1. No antibody: negative control without incubation with a primary antibody. (b) SRD5A2 was negatively correlated with DNMT1 expression in forty samples (Spearman's rank correlation, r = −0.340, P = 0.032). Scale bars = 50 μm. SRD5A2: 5-α reductase type 2; DNMT1: DNA methyltransferase 1.