Figure 6: Representative linear regression models indicating the negative correlation between sperm PLCζ variance from mice at 8 weeks, 12 weeks, 24 weeks, and 36 weeks of age, inclusive of both B6 and CD1 strains as indicated by (a) immunofluorescence and (b) immunoblotting. Representative histograms showing the relationship between sperm PLCζ variance from mice at 8 weeks, 12 weeks, 24 weeks, and 36 weeks of age from the B6 strain of mice individually as indicated by (c) immunofluorescence and (d) immunoblotting; and from the CD1 strain of mice individually as indicated by (e) immunofluorescence and (f) immunoblotting. Data are represented as the average standard deviation (s.d.) in relative fluorescence or normalized relative density (both in arbitrary units) ± s.e.m.*Statistically significant differences (P ≤ 0.05). Comparisons made are indicated by the respective line above the bars. NS: not significant; B6: C57BL/6 inbred mouse strain; CD1: outbred mouse strain; PLCζ: phospholipase C zeta; s.e.m: standard error of the mean.