Figure 2: Representative immunofluorescence images of observed PLCζ localization patterns in mouse sperm with relation to advancing male age, regardless of strain, at 8 weeks, 12 weeks, 24 weeks, and 36 weeks of age. Brightfield (left panel) and PLCζ (green fluorescence; right panel) images were obtained. PLCζ localization at the postacrosomal is indicated by red arrows, while acrosomal PLCζ is indicated by a yellow arrow. Fluorescence was also observed in the sperm tail throughout all ages examined. Images obtained were captured at ×100, scale bars = 5 μm. Images are representative of sperm from 6 animals from each age group, with a total of at least 600 cells examined per age group. Images are representative of each age group (i.e., the majority of sperm exhibited the depicted pattern(s) of localisaton. PLCζ: phospholipase C zeta.