Figure 1: (a) Representative immunoblotting analysis and corresponding ponceau-stained image indicating recognition of a single band corresponding to the molecular weight of PLCζ (approximately 74 kDa) in mouse sperm. (b) Representative immunofluorescence images of observed PLCζ localization patterns in mouse sperm. Brightfield (left panel) and PLCζ (green fluorescence; right panel) images were obtained. PLCζ localization was observed at the postacrosomal (red arrows) and acrosomal (yellow arrow) regions of the mouse sperm head. Fluorescence was also observed in the sperm tail. Images obtained were captured at ×100, scale bar = 5 μm. Quantification of PLCζ using (c) immunofluorescence and (d) immunoblotting did not indicate any significant differences in PLCζ from B6 or CD1 sperm, regardless of age. B6: C57BL/6 inbred mouse strain; CD1: outbred mouse strain; PLCζ: phospholipase C zeta; a.u.: arbitrary units.