Figure 5: Changes in nNOS-positive nerves in the corpus cavernosum at 1 week, 3 weeks, and 5 weeks after bilateral cavernous injury. (a) Representative immunofluorescence images of nNOS (red) and phalloidin (Pha, green) in 5-μm cross sections of cryo-embedded penile midshaft at ×400. (b) Comparison of semi-quantitative analysis of nNOS expression between treatment groups at each time point. *P < 0.05 means BCNR and Sham versus the BCNC group; #P < 0.05 means BCNC and Sham versus the BCNR group. (c) Comparison of nNOS expression within treatment groups at each time point. *P < 0.05 means week 1 and week 5 versus week 3; #P < 0.05 means week 1 and week 3 versus week 5. BCNR: bilateral cavernous nerve resection; BCNC: bilateral cavernous nerve crushing; nNOS: neuronal-nitric oxide synthase; DAPI: 4',6-diamidino-2-phenylindole.