Asian Journal of Andrology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 18  |  Issue : 3  |  Page : 485--491

Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis


Xing-Fa Han, Jun-Li Li, Yu-Qin Zhou, Xiao-Hua Ren, Gong-Cheng Liu, Xiao-Han Cao, Xiao-Gang Du, Xian-Yin Zeng 
 Isotope Research Biological Engineering and Application Biology Department, Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China

Correspondence Address:
Dr. Xian-Yin Zeng
Isotope Research Biological Engineering and Application Biology Department, Laboratory, Sichuan Agricultural University, Ya«SQ»an 625014
People«SQ»s Republic of China

GnRH sterilization vaccines have been developed for various practical and clinical reasons. However, conjugation of GnRH peptide to carrier protein has many drawbacks, hampering the further commercialization of GnRH vaccines. In this study, a new nonconjugated GnRH vaccine, D-Lys6-GnRH-tandem-dimer peptide (TDK), emulsified in Specol adjuvant was investigated for its immunocastration efficacy in young male rats. Prepubertal male rats were randomly allocated into three groups (n = 12): control (no treatment), surgically castrated or immunized against 100 μg TDK in Specol adjuvant at 6 weeks of age (with a booster 8 weeks later). Blood samples (for antibody titers and hormone concentrations) were collected at 2-week intervals until rats were killed (18 weeks of age). Compared to intact controls, active immunization against TDK reduced (P < 0.05) serum concentrations of testosterone, inhibin B, LH and FSH, prevented the onset of spermatogenesis at puberty. Furthermore, mRNA expressions of GnRH receptor, LH-β and FSH-β in the pituitary, LH receptor, FSH receptor, inhibin α, βA and βB subunit in the testes were decreased in immunocastrated rats compared to intact controls (P < 0.05). These results demonstrate for the first time that GnRH-tandem-dimer peptide emulsified in Specol is a promising veterinary sterilization medicine.


How to cite this article:
Han XF, Li JL, Zhou YQ, Ren XH, Liu GC, Cao XH, Du XG, Zeng XY. Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis.Asian J Androl 2016;18:485-491


How to cite this URL:
Han XF, Li JL, Zhou YQ, Ren XH, Liu GC, Cao XH, Du XG, Zeng XY. Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis. Asian J Androl [serial online] 2016 [cited 2021 Jan 20 ];18:485-491
Available from: https://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=3;spage=485;epage=491;aulast=Han;type=0