Asian Journal of Andrology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 18  |  Issue : 3  |  Page : 456--461

Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway


Ze He1, Qin Sun1, Yuan-Jiao Liang1, Li Chen1, Yi-Feng Ge1, Shi-Feng Yun2, Bing Yao1 
1 The Reproductive Medical Center, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China
2 Department of Comparative Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China

Correspondence Address:
Dr. Bing Yao
The Reproductive Medical Center, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002
China

This study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic acute regulatory (StAR), P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and 17α-hydroxylase were examined by Western blotting and semi-quantitative RT-PCR, respectively. Annexin A5 significantly stimulated testosterone secretion from rat Leydig cells in dose- and time-dependent manners and increased mRNA and protein expression of StAR, P450scc, 3β-HSD, and 17β-HSD but not 17α-hydroxylase. Annexin A5 knockdown by siRNA significantly decreased the level of testosterone and protein expression of P450scc, 3β-HSD, and 17β-HSD. The significant activation of ERK1/2 signaling was observed at 5, 10, and 30 min after annexin A5 treatment. After the pretreatment of Leydig cells with ERK inhibitor PD98059 (50 μmol l−1 ) for 20 min, the effects of annexin A5 on promoting testosterone secretion and increasing the expression of P450scc, 3β-HSD, and 17β-HSD were completely abrogated (P < 0.05). Thus, ERK1/2 signaling is involved in the roles of annexin A5 in mediating testosterone production and the expression of P450scc, 3β-HSD, and 17β-HSD in Leydig cells.


How to cite this article:
He Z, Sun Q, Liang YJ, Chen L, Ge YF, Yun SF, Yao B. Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway.Asian J Androl 2016;18:456-461


How to cite this URL:
He Z, Sun Q, Liang YJ, Chen L, Ge YF, Yun SF, Yao B. Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway. Asian J Androl [serial online] 2016 [cited 2021 Jan 21 ];18:456-461
Available from: https://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=3;spage=456;epage=461;aulast=He;type=0