Asian Journal of Andrology

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 17  |  Issue : 2  |  Page : 253--260

Alterations of gene profiles in Leydig-cell-regenerating adult rat testis after ethane dimethane sulfonate-treatment


Yu-Fei Zhang1, Kai-Ming Yuan2, Yong Liang3, Yan-Hui Chu1, Qing-Quan Lian2, Yu-Fei Ge4, Wei Zhen5, Chantal M Sottas4, Zhi-Jian Su6, Ren-Shan Ge7 
1 Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, Mudanjiang 157011, China
2 The 2nd Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China
3 The Affiliated Taizhou Municipal Hospital, Taizhou University, Taizhou 318000, China
4 The Population Council, New York 10065, USA
5 Department of Pathology, Molecular Pathology Laboratory, Montefiore Medical Centre, Albert Einstein College of Medicine, Bronx, NY 10467, USA
6 College of Life Science and Technology, Jinan University, Guangzhou 510632, China
7 The 2nd Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China; The Population Council, New York 10065, USA

Correspondence Address:
Ren-Shan Ge
The 2nd Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China; The Population Council, New York 10065, USA

Yan-Hui Chu
Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, Mudanjiang 157011, China

Only occupying about 1%-5% of total testicular cells, the adult Leydig cell (ALC) is a unique endocrine cell that produces androgens. Rat Leydig cells regenerate after these cells in the testis are eliminated with ethane dimethane sulfonate (EDS). In this study, we have characterized Leydig cell regeneration and messenger ribonucleic acids (mRNA) profiles of EDS treated rat testes. Serum testosterone, testicular gene profiling and some steroidogenesis-related proteins were analyzed at 7, 21, 35 and 90 days after EDS treatment. Testicular testosterone levels declined to undetectable levels until 7 days after treatment and then started to recover. Seven days after treatment, 81 mRNAs were down-regulated greater than or equal to two-fold, with 48 becoming undetectable. These genes increased their expression 21 days and completely returned to normal levels 90 days after treatment. The undetectable genes include steroidogenic pathway proteins: steroidogenic acute regulatory protein, Scarb1, Cyp11a1, Cyp17a1, Hsd3b1, Cyp1b1 and Cyp2a1. Seven days after treatment, there were 89 mRNAs up-regulated two-fold or more including Pkib. These up-regulated mRNAs returned to normal 90 days after treatment. Cyp2a1 did not start to recover until 35 days after treatment, indicating that this gene is only expressed in ALCs not in the precursor cells. Quantitative polymerase chain reaction, western blotting and semi-quantitative immunohistochemical staining using tissue array confirmed the changes of several randomly picked genes and their proteins.


How to cite this article:
Zhang YF, Yuan KM, Liang Y, Chu YH, Lian QQ, Ge YF, Zhen W, Sottas CM, Su ZJ, Ge RS. Alterations of gene profiles in Leydig-cell-regenerating adult rat testis after ethane dimethane sulfonate-treatment.Asian J Androl 2015;17:253-260


How to cite this URL:
Zhang YF, Yuan KM, Liang Y, Chu YH, Lian QQ, Ge YF, Zhen W, Sottas CM, Su ZJ, Ge RS. Alterations of gene profiles in Leydig-cell-regenerating adult rat testis after ethane dimethane sulfonate-treatment. Asian J Androl [serial online] 2015 [cited 2021 Sep 21 ];17:253-260
Available from: https://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=2;spage=253;epage=260;aulast=Zhang;type=0