Asian Journal of Andrology

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 16  |  Issue : 2  |  Page : 309--313

Bactericidal/permeability-increasing protein originates in both the testis and the epididymis and localizes in mouse spermatozoa


Zhong-Ping Zhou, Xiao-Yu Xia, Qiang-Su Guo, Chen Xu 
 Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Reproductive Medicine, Shanghai, China

Correspondence Address:
Chen Xu
Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Reproductive Medicine, Shanghai
China

Bactericidal/permeability-increasing protein (BPI) is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes and epididymides as well as the subcellular localization within epididymal spermatozoa. Our results showed that, BPI mRNA was expressed in testis and epididymis independently. Throughout the epididymis, the BPI protein level gradually decreased in the epididymal epithelium in a spatial manner, specialized within the cytoplasm of clear cells in the cauda part. We detected BPI proteins in intact acrosome, implying its testicular origin; on the other hand, after the acrosome reaction, BPI proteins were observed dispersed across the entire sperm head, especially enriched at the equatorial segment. Our findings suggested a dual origin of the BPI that generated both in the testis and epididymis, and associated with mouse spermatozoa. BPI protein might be involved in the dynamics modification of the sperm plasma membrane and also the fertilization process.


How to cite this article:
Zhou ZP, Xia XY, Guo QS, Xu C. Bactericidal/permeability-increasing protein originates in both the testis and the epididymis and localizes in mouse spermatozoa.Asian J Androl 2014;16:309-313


How to cite this URL:
Zhou ZP, Xia XY, Guo QS, Xu C. Bactericidal/permeability-increasing protein originates in both the testis and the epididymis and localizes in mouse spermatozoa. Asian J Androl [serial online] 2014 [cited 2021 Sep 28 ];16:309-313
Available from: https://www.ajandrology.com/article.asp?issn=1008-682X;year=2014;volume=16;issue=2;spage=309;epage=313;aulast=Zhou;type=0