Figure 6: Detection of oxidative stress by cavernous Nrf2, HO-1 immunohistochemical staining and western blot. (a) Cavernous Nrf2 immunohistochemical staining; (b) bar graph of Nrf2 positive area; (c) cavernous HO-1 immunohistochemical staining; (d) bar graph of HO-1 positive area; (e) western blot of the Nrf2/Keap-1 pathways; (f) bar graph of relative Nrf2/Keap-1 expression; (g) bar graph of relative HO-1 expression; scale bars = 50 μm; n = 6 in each group. Representative micrographs of cavernous Nrf2 immunohistochemical staining show areas of oxidative stress as brown (×200). Representative micrographs of cavernous HO-1 immunohistochemical staining show areas of oxidative stress as brown (×200). Data of western blot analysis are shown as the fold changes over the control group. Bar graphs represent mean ± standard deviation. *P < 0.05 and **P < 0.01, the indicated group compared with the Sham group; #P < 0.05 and ##P < 0.01, the indicated group compared with the BCNI group. BCNI: bilateral cavernous nerve injury; HO-1: heme oxygenase-1; Keap-1: Kelch-like ECH associated protein 1; Nrf2: nuclear factor erythroid 2-related factor 2.