Figure 2: Dot plots obtained on the C6 Plus with the modified or adjusted gates for negative control sperm sample. The first plot a depicts the FSC-A versus SSC-A plot with flame shaped gate that captures the spermatozoa and apoptotic bodies, plot b is a PI-A/FSC-A plot with PI positive events. This gate includes all spermatozoa and excludes the M450 apoptotic bodies, (c) is the PI-A/FITC-A plot for the negative sperm control which is used to set the quadrants, (d) depicts the PI-A versus FITC-A gate plot of a negative test sample. The positive sperm sample was run in duplicate, and shows SDF of (e) 36.7% and (f) 36.1 %. The SDF values of the test sample run in duplicate reflect the very low intra-assay variability. FITC-A: fluorescein isothiocynate-area; SSC-A: side scatter-area; PI: propidium iodide; SDF: sperm DNA fragmentation; UL: upper left; UR: upper right; LL: lower left; LR: lower right; P2: gate P2; P4: gate P4; Q1: quadrant 1.