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  Indian J Med Microbiol
 

Figure 1: Representative dot plots on the C6 Plus flow cytometer showing comparison of data acquisition utilizing unadjusted and adjusted settings with data acquisition images of positive sample with (a) unadjusted flame shaped gate settings on C6 Plus flow cytometer (FSC-A vs SSC-A), (b) unadjusted settings on C6 Plus flow cytometer (FSC-A vs PI), (c) unadjusted settings on C6 Plus flow cytometer (FITC-A vs PI), (d) adjusted settings on C6 Plus flow cytometer (FSC-A vs SSC-A) including the sperm and the apoptotic bodies. (e) Data plots of positive sperm sample with the adjusted settings on C6 Plus flow cytometer (FSC-A vs PI) including the PI-positive events within the gate and excluding the M450 apoptotic bodies. (f) Data acquisition images of positive sperm sample with the adjusted settings on C6 Plus flow cytometer (FITC-A vs PI): the TUNEL/PI-positive events are depicted in the right upper quadrant and this estimates the TUNEL positivity. FITC-A: fluorescein isothiocynate-area; SSC-A: side scatter area; PI: propidium iodide; UL: upper left; UR: upper right; LL: lower left; LR: lower right; P1: gate P1; P3: gate P3; Q2: quadrant 2.

Figure 1: Representative dot plots on the C6 Plus flow cytometer showing comparison of data acquisition utilizing unadjusted and adjusted settings with data acquisition images of positive sample with (<b>a</b>) unadjusted flame shaped gate settings on C6 Plus flow cytometer (FSC-A <i>vs</i> SSC-A), (<b>b</b>) unadjusted settings on C6 Plus flow cytometer (FSC-A <i>vs</i> PI), (<b>c</b>) unadjusted settings on C6 Plus flow cytometer (FITC-A <i>vs</i> PI), (<b>d</b>) adjusted settings on C6 Plus flow cytometer (FSC-A <i>vs</i> SSC-A) including the sperm and the apoptotic bodies. (<b>e</b>) Data plots of positive sperm sample with the adjusted settings on C6 Plus flow cytometer (FSC-A <i>vs</i> PI) including the PI-positive events within the gate and excluding the M450 apoptotic bodies. (<b>f</b>) Data acquisition images of positive sperm sample with the adjusted settings on C6 Plus flow cytometer (FITC-A <i>vs</i> PI): the TUNEL/PI-positive events are depicted in the right upper quadrant and this estimates the TUNEL positivity. FITC-A: fluorescein isothiocynate-area; SSC-A: side scatter area; PI: propidium iodide; UL: upper left; UR: upper right; LL: lower left; LR: lower right; P1: gate P1; P3: gate P3; Q2: quadrant 2.