Figure 6: The effect of voltage-dependent calcium channel of sperm - CatSper inhibition on AE. After 160 min of incubation (Control), 1 μmol l−1 CatSper inhibitor (1S,2S)-2-(2-(N-[(3-Benzimidazol-2-yl)propyl]-N-methylamino)ethyl)-6-fluoro-1,2,3,4-tetrahydro-1-isopropyl-2-naphtyl cyclopropanecarboxylate dihydrochloride hydrate (NNC 55-0396) were added and then the AE inducers N-[2-(p-Bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89; 50 μmol l−1), TG (3 μmol l−1), progesterone (5 μmol l−1), 8-(4-Chlorophenylthio)-2'-O-methyladenosine-3′,5′-cyclic (8pCPT; 0.1 μmol l−1), or antibiotic A23187 calcium ionophore (A23187; 10 μmol l−1) were added. The values represent the mean ± standard deviation of duplicates from three experiments from three different donors.*P < 0.05,**P < 0.01, and***P < 0.001, significant difference compared to the corresponding control. AE: acrosomal exocytosis; TG: thapsigargin.