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  Indian J Med Microbiol
 

Figure 5: The actin florescence intensity. Human sperm (1 × 107 cells per ml) were incubated in Ham's F-10 capacitation medium for 160 min (Control). Next, 10 μmol l−1 EPAC specific inhibitor 09 (ESI09) was added for an additional 20 min. Then TG (3 μmol l−1), polyphosphoinositide-binding-peptide (PBP10; 1 μmol l−1), N-[2-(p-bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89; 50 μmol l−1), 8-(4-Chlorophenylthio)-2′-O-methuladenosine-3′,5′-cyclic (8pCPT; 0.1 μmol l−1] were added for an additional 1 h. The values represent the mean ± standard deviation of duplicates from two experiments from two different donors. (a) F-actin fluorescence units. (b) Fluorescence microscope figures. Scale bars = 5 μm. EPAC: exchange protein directly activated by cAMP; cAMP: cyclic adenosine monophosphate; TG: thapsigargin.

Figure 5: The actin florescence intensity. Human sperm (1 × 10<sup>7</sup> cells per ml) were incubated in Ham's F-10 capacitation medium for 160 min (Control). Next, 10 μmol l<sup>−1</sup> EPAC specific inhibitor 09 (ESI09) was added for an additional 20 min. Then TG (3 μmol l<sup>−1</sup>), polyphosphoinositide-binding-peptide (PBP10; 1 μmol l<sup>−1</sup>), N-[2-(p-bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89; 50 μmol l<sup>−1</sup>), 8-(4-Chlorophenylthio)-2′-O-methuladenosine-3′,5′-cyclic (8pCPT; 0.1 μmol l<sup>−1</sup>] were added for an additional 1 h. The values represent the mean ± standard deviation of duplicates from two experiments from two different donors. (<b>a</b>)  F-actin fluorescence units. (<b>b</b>)  Fluorescence microscope figures. Scale bars = 5 μm. EPAC: exchange protein directly activated by cAMP; cAMP: cyclic adenosine monophosphate; TG: thapsigargin.