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  Indian J Med Microbiol
 

Figure 3: Inhibitory effect of the phospholipase C inhibitor 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione (U73122) on AE. After 160 min of incubation (Control), 1 μmol l−1 U73122 (PLC inhibitor) or 1 μmol l−1 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-2,5-pyrrolidinedione (U73343, inactive PLC inhibitor analogue) were added and then the AE inducers N-[2-(p-bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89; 50 μmol l−1), TG (3 μmol l−1), progesterone (5 μmol l−1), 8-(4-Chlorophenylthio)-2'-O-methyladenosine -3′,5′-cyclic (8pCPT; 0.1 μmol l−1) or antibiotic A23187 Calcium Ionophore (A23187; 10 μmol l−1) were added. The values represent the mean ± standard deviation of duplicates from three experiments from three different donors.*P < 0.05,**P < 0.01, and***P < 0.001, significant difference compared to the corresponding control. PLC: phospholipase C; AE: acrosomal exocytosis; TG: thapsigargin.

Figure 3: Inhibitory effect of the phospholipase C inhibitor 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione (U73122) on AE. After 160 min of incubation (Control), 1 μmol l<sup>−1</sup> U73122 (PLC inhibitor) or 1 μmol l<sup>−1</sup> 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-2,5-pyrrolidinedione (U73343, inactive PLC inhibitor analogue) were added and then the AE inducers N-[2-(p-bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89; 50 μmol l<sup>−1</sup>), TG (3 μmol l<sup>−1</sup>), progesterone (5 μmol l<sup>−1</sup>), 8-(4-Chlorophenylthio)-2'-O-methyladenosine -3′,5′-cyclic (8pCPT; 0.1 μmol l<sup>−1</sup>) or antibiotic A23187 Calcium Ionophore (A23187; 10 μmol l<sup>−1</sup>) were added. The values represent the mean ± standard deviation of duplicates from three experiments from three different donors.<sup>*</sup><i>P</i> < 0.05,<sup>**</sup><i>P</i> < 0.01, and<sup>***</sup><i>P</i> < 0.001, significant difference compared to the corresponding control. PLC: phospholipase C; AE: acrosomal exocytosis; TG: thapsigargin.