Figure 2: The effect of 8-(4-Chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic (8pCPT) on AE induced by protein kinase A inhibition. After 160 min of incubation (Control), various concentrations of 8pCPT were added and then 50 μmol l−1 N-[2-(p-bromocinnamylamino)ethyl]-5isoquinolinesulfonamide (H89) or 0.5 mmol l−1 1-methyl-3-isobutylxanthine (IBMX) were added. The values represent the mean ± standard deviation of duplicates from three experiments from three different donors.*P < 0.05,**P < 0.01, and***P < 0.001, significant difference compared to the corresponding control. AE: acrosomal exocytosis.