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  Indian J Med Microbiol
 

Figure 5: Effect of SBNO1 deficiency on the noncanonical Wnt pathway. The relative transcript levels of (a) Wnt5b, (b) Wnt2b, (c) Wnt5a, (d) Wnt11 and (e) Wnt6 in Ctr and Mo testes. (f) Western blot analysis showing a decrease in p-JNK in Mo group, and no change in total JNK levels. (g) Representative images of β-catenin (green) immunostaining in Ctr and Mo testes. ***P < 0.001, Student's t-test, n = 3. Scale bar = 20 μm. Ctr: negative control; Mo: morpholino treatment; Wnt-5b: wingless-type MMTV integration site family member 5B; Wnt-2b: wingless-type MMTV integration site family member 2B; Wnt-5a: wingless-type MMTV integration site family member 5A; Wnt-11: wingless-type MMTV integration site family member 11; Wnt-6: wingless-type MMTV integration site family member 6; p-JNK: phosphorylated form of Jun N-terminal kinase; DAPI: 4',6-diamidino-2-phenylindole; MMTV: murine mammary tumor virus.

Figure 5: Effect of SBNO1 deficiency on the noncanonical Wnt pathway. The relative transcript levels of (<b>a</b>) <i>Wnt5b</i>, (<b>b</b>) <i>Wnt2b</i>, (<b>c</b>) <i>Wnt5a</i>, (<b>d</b>) <i>Wnt11</i> and (<b>e</b>) <i>Wnt6</i> in Ctr and Mo testes. (<b>f</b>) Western blot analysis showing a decrease in p-JNK in Mo group, and no change in total JNK levels. (<b>g</b>) Representative images of β-catenin (green) immunostaining in Ctr and Mo testes. <sup>***</sup><i>P</i> < 0.001, Student's <i>t</i>-test, <i>n</i> = 3. Scale bar = 20 μm. Ctr: negative control; Mo: morpholino treatment; <i>Wnt-5b</i>: wingless-type MMTV integration site family member 5B; <i>Wnt-2b</i>: wingless-type MMTV integration site family member 2B; <i>Wnt-5a</i>: wingless-type MMTV integration site family member 5A; <i>Wnt-11</i>: wingless-type MMTV integration site family member 11; <i>Wnt-6</i>: wingless-type MMTV integration site family member 6; p-JNK: phosphorylated form of Jun N-terminal kinase; DAPI: 4',6-diamidino-2-phenylindole; MMTV: murine mammary tumor virus.