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  Indian J Med Microbiol
 

Figure 3: SBNO1 and germ cell proliferation. (a) Representative images of KI67 (proliferative cell marker, red) immunostaining in Ctr and Mo testes. (b) Quantification of a. (c) Representative images of PH3 (proliferative cell marker, red) immunostaining in Ctr and Mo testes. (d) Quantification of c. (e) Representative images showing the results of the TUNEL assay (red) in Ctr and Mo testes. (f) Quantification of e. **P < 0.01, Student's t-test, n = 3. Scale bars = 20 μm. Ctr: negative control; Mo: morpholino-treatment; KI67: marker of proliferation Ki-67; PH3: phospho-histone H3; TUNEL: terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick-end labeling; DAPI: 4',6-diamidino-2-phenylindole.

Figure 3: SBNO1 and germ cell proliferation. (<b>a</b>) Representative images of KI67 (proliferative cell marker, red) immunostaining in Ctr and Mo testes. (<b>b</b>) Quantification of <b>a</b>. (<b>c</b>) Representative images of PH3 (proliferative cell marker, red) immunostaining in Ctr and Mo testes. (<b>d</b>) Quantification of <b>c</b>. (<b>e</b>) Representative images showing the results of the TUNEL assay (red) in Ctr and Mo testes. (<b>f</b>) Quantification of <b>e</b>. <sup>**</sup><i>P</i> < 0.01, Student's <i>t</i>-test, <i>n</i> = 3. Scale bars = 20 μm. Ctr: negative control; Mo: morpholino-treatment; KI67: marker of proliferation Ki-67; PH3: phospho-histone H3; TUNEL: terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick-end labeling; DAPI: 4',6-diamidino-2-phenylindole.