Figure 2: SBNO1 and germ cell development. (a) Western blot analysis shows high knockdown efficiency of SBNO1 by morpholino. (b) Quantification of a. (c) Representative images of DDX4 (germ cell marker, red) immunostaining in Ctr and Mo testes. (d) Quantification of c. (e) Representative images of LIN28 (undifferentiated spermatogonia marker, red) immunostaining in Ctr and Mo testes. (f) Quantification of e. ***P < 0.001, Student's t-test, n = 3. Scale bars = 20 μm. Ctr: negative control; Mo: morpholino-treatment; SBNO1: Strawberry Notch 1; DDX4: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; LIN28: lin-28 homolog; DAPI: 4',6-diamidino-2-phenylindole.