Figure 1: Expression and distribution of SBNO1 in neonatal testes and SSCs. (a) Representative images of SBNO1 (red) immunostaining in 3.5-day, 5.5-day and 7.5-day mouse testes. (b) Co-immunostaining of SBNO1 (red) and PLZF (undifferentiated spermatogonia marker, green) in 5.5-day mouse testes. (c) Immunofluorescence of SBNO1 (red) and PLZF (green) in SSCs. Scale bars = 20 μm. SBNO1: Strawberry Notch 1; PLZF: promyelocytic leukemia zinc finger; DAPI: 4',6-diamidino-2-phenylindole; SSC: spermatogonial stem cell.