Figure 1: Lycopene dose/time-dependent suppressed the viability and increased the apoptosis capacity of prostatic carcinoma cancer cell lines (LNCaP, PC3, and DU145 cells). (a) Prostatic carcinoma cell lines were placed on 96-well plates (1000 cells per well) and incubated with fresh medium containing 0, 0.1, 0.5, 1, or 5 μmol l−1 lycopene for 0, 24, 48 and 72 h. Viability of individual treated cell lines and non-treated cell lines were detected by CCK-8 kit. Absorbance was measured at 450 nm and the cell viability was represented as: cell viability (%) = (OD [treated] − OD [0 h])/OD (0 h) × 100%. (b) Lycopene induces prostatic carcinoma cancer cell apoptosis. Annexin V/propidium iodide double-staining assay was performed to detect the apoptosis levels of lycopene-treated or not prostatic carcinoma cell lines at the indicated concentrations for 72 h. Relative expression values represent mean and standard deviation from three independent experiments. The apoptosis levels of untreated cells were used to normalize those of the treated cells and the data were represented in percentages. *P < 0.01, groups treated with lycopene versus control (0 μmol l-1). OD: optical density; CCK-8: cell counting kit-8.