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  Indian J Med Microbiol
 

Figure 5: Effect of GRK2 inhibition on oxidative stress. (a) The expression of gp91phox mRNA was measured by real-time quantitative polymerase chain reaction (PCR). The data were presented as the fold change relative to the non-diabetic controls. (b) gp91phox protein expression was measured by western blot analysis. β-actin was used as a loading control. (c) Expression levels of gp91phox protein were normalized to β-actin. (d) ROS production in cavernosal tissue was expressed as CPM mg-1 protein. Results were reported as mean ± standard deviation (s.d.). *P < 0.05, the indicated group versus control group; ‘P < 0.05, the indicated group versus T2DM+vehicle group; n = 20 per group. All results are representative of three independent experiments. CPM: counts per min; GRK2: G protein-coupled receptor kinase 2; T2DM: type 2 diabetes mellitus; ROS: reactive oxygen species.

Figure 5: Effect of GRK2 inhibition on oxidative stress. (<b>a</b>) The expression of gp91<sup>phox</sup> mRNA was measured by real-time quantitative polymerase chain reaction (PCR). The data were presented as the fold change relative to the non-diabetic controls. (<b>b</b>) gp91<sup>phox</sup> protein expression was measured by western blot analysis. β-actin was used as a loading control. (<b>c</b>) Expression levels of gp91<sup>phox</sup> protein were normalized to β-actin. (<b>d</b>) ROS production in cavernosal tissue was expressed as CPM mg<sup>-1</sup> protein. Results were reported as mean ± standard deviation (s.d.). *<i>P</i> < 0.05, the indicated group versus control group; ‘<i>P</i> < 0.05, the indicated group versus T2DM+vehicle group; <i>n</i> = 20 per group. All results are representative of three independent experiments. CPM: counts per min; GRK2: G protein-coupled receptor kinase 2; T2DM: type 2 diabetes mellitus; ROS: reactive oxygen species.