Figure 2: CHRM1 expression in prostate cancer cells. (a) Western blotting images of CHRM1 in RWPE-1, LNCaP, and PC-3 cells. (b) Western blotting images of CHRM1 in PC-3, LNCaP, and A549 cells. Immunohistochemical staining of CHRM1 in (c) PC-3, (d) LNCaP, and (e) A549 cell lines. Scale bars = 100 μm. (f) Pirenzepine at 100–140 μg ml-1 inhibited PC-3 cell proliferation in a concentration-dependent manner. (g) Carbachol promoted PC-3 cell proliferation at 2–10 μg ml-1 even though these changes did not reach statistical significance. Each concentration was tested in five replicates. The results are shown as the mean ± s.e.m. CHRM1: muscarinic acetylcholine receptor M1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; s.e.m.: standard error of the mean.