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  Indian J Med Microbiol
 

Figure 2: CHRM1 expression in prostate cancer cells. (a) Western blotting images of CHRM1 in RWPE-1, LNCaP, and PC-3 cells. (b) Western blotting images of CHRM1 in PC-3, LNCaP, and A549 cells. Immunohistochemical staining of CHRM1 in (c) PC-3, (d) LNCaP, and (e) A549 cell lines. Scale bars = 100 μm. (f) Pirenzepine at 100–140 μg ml-1 inhibited PC-3 cell proliferation in a concentration-dependent manner. (g) Carbachol promoted PC-3 cell proliferation at 2–10 μg ml-1 even though these changes did not reach statistical significance. Each concentration was tested in five replicates. The results are shown as the mean ± s.e.m. CHRM1: muscarinic acetylcholine receptor M1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; s.e.m.: standard error of the mean.

Figure 2: CHRM1 expression in prostate cancer cells. (<b>a</b>) Western blotting images of CHRM1 in RWPE-1, LNCaP, and PC-3 cells. (<b>b</b>) Western blotting images of CHRM1 in PC-3, LNCaP, and A549 cells. Immunohistochemical staining of CHRM1 in (<b>c</b>) PC-3, (<b>d</b>) LNCaP, and (<b>e</b>) A549 cell lines. Scale bars = 100 μm. (<b>f</b>) Pirenzepine at 100–140 μg ml<sup>-1</sup> inhibited PC-3 cell proliferation in a concentration-dependent manner. (<b>g</b>) Carbachol promoted PC-3 cell proliferation at 2–10 μg ml<sup>-1</sup> even though these changes did not reach statistical significance. Each concentration was tested in five replicates. The results are shown as the mean ± s.e.m. CHRM1: muscarinic acetylcholine receptor M1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; s.e.m.: standard error of the mean.