Figure 2: Graphic representation of embryo quality on day 5 and its association with sperm tACE. Embryo development phase at the blastocyst stage and the association with tACE. (a) Number of tACE molecules per spermatozoon and (b) percentage of tACE-positive spermatozoa. Blastocyst viability on day 5 of development and association with tACE: (c) number of tACE molecules per spermatozoon and (d) percentage of tACE-positive spermatozoa. MC: compact morula stage; BT: early blastocyst; BC: expanding blastocyst; BE: expanded blastocyst; BHi: hatching/hatched blastocyst; BD: blocked or degenerated embryos; V: viable blastocyst; NV: non-viable blastocyst. Box-plot graph shows the median values as lines across the box. Lower and upper box lines indicate the 25th–75th percentile. Whiskers represent the maximum and minimum values. *P < 0.05 and **P < 0.01. tACE: testicular angiotensin-converting enzyme.