Figure 4: Proliferation and clonogenic assays. (a) Clonogenic assay of 22Rv1 cells with ZEB1 overexpression and DU145 cells with ZEB1 silencing compared to their controls. Photographs from representative experiments are shown. (b) Graphical representation from the results of the clonogenic assays in (a). (c) Proliferation curves of 22Rv1 cells with overexpressing ZEB1 and DU145 with ZEB1 silencing. n = 3, *P < 0.05 in all cases.